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OLEH V. LUSHCHAK, PhD


ADDRESS
Address: Department of Biochemistry and Biotechnology, Natural Sciences Institute,
            Vassyl Stefanyk Precarpathian University, Ivano-Frankivsk, Ukraine
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PERSONAL
Ukrainian, male, born May 22, 1983 (Moscow).
Home address: 16 Stefanyka Str., Lysets, Ivano-Frankivsk Distr., 77455, Ukraine
Languages – English, Ukrainian, Russian  

EDUCATION
2000-2005 – Student in Department of Biochemistry and Biotechnology, Natural Sciences Institute,
            Vassyl Stefanyk Precarpathian University, Ivano-Frankivsk, Ukraine
2004 – Bachelor’s degree in Biochemistry
2005 – Master’s degree in Biochemistry
2005-2008 – Ph.D. student Department of Biochemistry and Biotechnology
2009 – PhD degree in Biochemistry

RESEARCH EXPERIENCE AND PRACTICAL TRAININGS
2000-11 – Department of Biochemistry and Biotechnology, Vassyl Stefanyk Precarpathian   
University, Ivano-Frankivsk, Ukraine
2002 – Training in Marine Biology, Institute of Marine Biology, Kotor, Montenegro
2003 – Department of Biophysics, Otto-von-Gurike University, Magdeburg, Germany
2009 – Department of Neurobiology and Genetics, University of Wurzburg, Wurzburg, Germany
2009 – Buck Institute for Age Research, Novato, CA, USA
2010 – Department of Neurobiology and Genetics, University of Wurzburg, Wurzburg, Germany
2011-13 – Department of Zoology, Stockholm University, Stockholm, Sweden
2012 – Institute of Biomedical Technology, University of Tampere, Tampere, Finland


CURRENT AREAS OF INTEREST
    Neuronal regulation of metabolism;
    Aging and age-related diseases;
    Signaling in regulation of aging;
    Nutrition, mitochondria and ROS metabolism;

CURRENT RESEARCH WORK
    Smell in regulation of metabolism and lifespan;
    Regulation of metabolism by odor sensitive neurons;
    Role of serotonin and octopamine receptors in regulation of insulin signaling;
    Interaction between insulin and AKH in regulation of metabolism;
    Insulin in regulation of food intake and metabolism.


Awards AND GRANTS
October 2008 – Travel Fellowship grant from Journal of Experimental Biology to visit Dr Bertram Gerber.
August 2008 – Participation in 12th Yeast Congress, Kyiv, Ukraine. Was selected to oral presentation of the results. Participation fee was covered by FEMS.
September 2007 – Participation in international Workshop:  Molecular physiology of membrane transport and cellular signaling, Yaremche, Ukraine. Participation fee was covered by The Physiological Society
2007 – First place for the best oral presentation in young scientists' conference "Youth and progress in biology" (Lviv, Ukraine).
September-October 2006 – Participation in International Free Radical Summer School 2006: Biomarkers of oxidative stress and responses, Spetses Island, Greece. Participation fee and travel grants were provided by FEBS.
October 2006 – Award for the best poster in "IX Congress of Ukrainian Biochemical Society".
2005 – Second place for the best oral presentation in young scientists' conference "Youth and progress in biology" (Lviv, Ukraine).
2003 – DAAD grant for student exchange, training in Biophysics, Otto-von-Gurike University, Magdeburg, Germany.
2002 – INTAS funded program for student exchange, training in Marine Biology, Institute of Marine Biology, Kotor, Montenegro.

honours
2012 – Who's Who in the World
2012 – 2000 Outstanding Intellectuals of the 21st Century 2012

Editorial boards
2012 – present Biochemistry and Physiology
2012 – present Biochemistry and Pharmacology
autor


PUBLICATIONS

* - corresponding author
** - co-corresponding author

Articles

36. Gospodaryov D., Yurkevych I., Lushchak O**., Lushchak V. Lifespan extension and delay of age-related functional decline caused by Rhodiola rosea depends on dietary macronutrient balance. Longevity & Healthspan, 2013, 2:12.
35. Nässel D., Kubrak O., Liu Y., Luo J., Lushchak O. Factors that regulate insulin producing cells and their output in Drosophila. Front. Physiol., 2013, in press.
34. Lushchak O*., Gospodaryov D., Rovenko B., Yurkevych I., Perkhulyn N., Lushchak V. Specific Dietary Carbohydrates Differentially Influence the Life Span and Fecundity of Drosophila melanogaster. J Gerontol A Biol Sci Med Sci., 2013, in press.
33. Lozinsky O., Lushchak O., Kryshchuk N., Shchypanska N., Riabkina A., Skarbek S., Maksymiv I., Storey J., Storey K., Lushchak V. S-nitrosoglutathione-induced toxicity in Drosophila melanogaster: Delayed pupation and induced mild oxidative/nitrosative stress in eclosed flies. Comp. Biochem. Physiol., 2013, 164(1), 162-170.

32. Kapan N., Lushchak O., Luo J., Nässel D. Identified peptidergic neurons in the Drosophila brain regulate insulin-producing cells, stress responses and metabolism by coexpressed short neuropeptide F and corazonin. Cell. Mol. Life Sci., 2012, 69(23), 4051-4066

31. Rovenko B., Lushchak O*., Lozinsky O.,  Kubrak O., Lushchak V. Mild oxidative stress in fruit fly Drosophila melanogaster induced by sucrose. Ukr. Biochem. J., 2012, 84 (5), 97-105.

30. Lozinsky O., Lushchak O., Lushchak V. Sodium nitroprusside toxicity in Drosophila melanogaster: delayed pupation, reduced adult emergence, and induced oxidative/nitrosative stress in eclosed flies. Arch. Insect Biochem. Physiol., 2012, 80(3), 166-185.
29. Lushchak O*., Gospodaryov D., Rovenko B., Glovyak A., Yurkevych I., Klyuba V., Shcherbij M., Lushchak V. Balance between macronutrients affects life span and functional senescence in fruit fly Drosophila melanogaster. J. Gerontol. A Biol. Sci., 2012, 67(2), 118-125.
28. Lushchak O., Rovenko B., Gospodaryov D., Lushchak V. Drosophila melanogaster larvae fed by glucose and fructose demonstrate difference in oxidative stress markers and antioxidant enzymes of adult flies. Comp. Biochem. Physiol., 2011, 160(1), 27-34.
27. Kubrak O., Lushchak O., Lushchak J., Torous I., Storey J., Storey K., Lushchak V. Chromium effects on free radical processes in goldfish tissues: Comparison of Cr(III) and Cr(VI) exposures on oxidative stress markers, glutathione status and antioxidant enzymes. Comp. Biochem. Physiol., 2010, 152, 360-370.
26. Lushchak O., Inoue Y., Lushchak V. Saccharomyces cerevisiae exhibits a Yap1-mediated adaptive response to SNP-induced stress. Biochemistry (Moscow), 2010, 75(5), 629-635.
25. Lushchak O., Bahniukova T., Lushchak V. Activation of enzymes of soxRS-regulon by hydrogen peroxide in Escherichia coli.  Microbiol. J., 2009, 71 (1), 22-27
24. Lushchak O., Bayliak M., Korobova O., Levine R., Lushchak V. Buffer modulation of menadione-induced oxidative stress in Saccharomyces cerevisiae. Redox Report, 2009, 14(5), 214-220.
23. Lushchak O., Kubrak O., Storey J., Storey K., Lushchak V. Low toxic herbicide Roundup induces mild oxidative stress in goldfish tissues. Chemosphere, 2009, 76 (7), 932-937.
22. Lushchak O., Kubrak O., Lozinsky O., Storey J., Storey K., Lushchak V. Chromium(III) induces oxidative stress in goldfish liver and kidney. Aquat. Toxic., 2009, 93, 45–52.
21. Lushchak O., Lushchak V. Possible pathways involved in activation of catalase and superoxide dismutase in yeast S. сerevisiae treated with sodium nitroprusside. Ukr. Biochem. J., 2009, 81 (2), 26-31.
20. Lushchak O., Kubrak O., Torous I., Nazarchuk T., Storey K., Lushchak V. Trivalent chromium induces oxidative stress in goldfish brain. Chemosphere, 2009, 75, 56-62.
19.    Semchuk N., Lushchak O., Nazarchuk T., Falk J., Krupinska K., Lushchak V. Inactivation of genes, encoding tocopherol biosynthesis pathways enzymes, results in oxidative stress in outdoor grown Arabidopsis thaliana. Plant Physiol. Biochem., 2009, 47(5), 384-390.
18. Lushchak O., Nykorak M., Ohdat T., Inoue Y., Lushchak V. Inactivation of superoxide dismutase incoding genes modifies yeast cell response to S-nitrosoglutathione induced stress. Biochemistry (Moscow), 2009, 74(4), 550-557.
17. Lushchak O., Lushchak V. Catalase modifies yeast Saccharomyces cerevisiae response toward S-nitrosoglutathione-induced stress. Redox Report, 2008, 13(6), 283-291.
16. Semchuk N., Lushchak O., Falk J., Krupinska K., Lushchak V. Effects of VTE1 and VTE4 gene inactivation of salt stress response in Arabidopsis thaliana. Ukr. Biochem. J., 2008, 80 (3), 48-54.
15. Lushchak O., Kubrak O., Nykorak M., Storey K., Lushchak V. The effect of potassium dichromate on free radical processes in goldfish: Possible protective role of glutathione. Aquat. Toxic., 2008, 87, 108–114.
14. Lushchak O., Lushchak V. Sodium nitroprusside induces mild oxidative stress in Saccharomyces cerevisiae. Redox Report, 2008, 13(4), 144-152.
13. Lushchak O., Lozinsky O., Nazarchuk T., Lushchak V. S-nitrosoglutathione induces nitrosative stress in yeast: modified role of catalase. Ukr. Biochem. J., 2008, 80 (2), 84-91.
12. Kubrak O., Lushchak O., Lushchak V. Comparative characteristics of lactate dehydrogenase from the liver and white muscles of common carp (Cyprinus carpio). Ukr. Biochem. J., 2008, 80 (4), 35-41.
11. Lushchak O., Semchyshyn H., Lushchak V. Coordinated yeast S. cerevisiae cell response on inactivation of genes encoding superoxide dismutases. Redox Report, 2007, 12(4), 181-188.
10. Lushchak V., Bagnyukova T., Lushchak O., Storey J., Storey K. Superoxide dismutase inhibition by diethyldithiocarbamate induces oxidative stress in goldfish brain. Chem.-Biol. Interact., 2007, 170(1), 1-8.
 9. Bagnyukova T., Lushchak O., Storey K., Lushchak V., Oxidative stress and antioxidant defense responses by goldfish tissues to acute change of temperature from 3 to 23ºC. J. Thermal Biol., 2007, 32, 227–234
 8. Mandryk S., Lushchak O., Semchyshyn H., Lushchak V. Role of Cu,Zn- and Mn-containing superoxide dismutases in yeast cell during growth on ethanol. Microbiol. J. (Ukr), 2007, 69(2), 35-42.
 7. Lushchak O*., Muller S., Mair T. Synchronization of NADH oscillations in yeasts Saccharomyces cerevisiae and S. carlsbergensis. Ukr. Biochem. J., 2006, 78 (5), 12-18.
 6. Lushchak V., Semchyshyn H., Mandryk S., Lushchak O. Possible role of superoxide dismutases in the yeast Saccharomyces cerevisiae under respiratory conditions. Arch. Biochem. Biophys., 2005, 441, 35-40.
 5. Lushchak V., Semchyshyn H., Lushchak O., Mandryk S. Diethyldithiocarbamate inhibits in vivo Cu,Zn-superoxide dismutase and perturbs free radical processes in the yeast Saccharomyces cerevisiae cells. Biochem. Biophys. Res. Commun., 2005, 338, 1739-1744.
 4. Lushchak V., Bagnyukova T., Husak V., Luzhna L., Lushchak O., Storey K. The effect of hyperoxia on free radical processes in goldfish tissues. Int. J. Biochem. Cell Biol., 2005, 37, 1670-1680.
 3. Lushchak V., Bagnyukova T., Lushchak O., Storey J., Storey K. Hypoxia and recovery perturb free radical processes and antioxidant potential in common carp (Cyprinus carpio). Int. J. Biochem. Cell Biol., 2005, 37, 1319-1330.
 2. Lushchak V., Bagnyukova T., Lushchak O. Indices of oxidative stress. 1. TBA-reactive substances and carbonylproteins. Ukr. Biochem. J., 2004, 76 (3), 136-141.
 1. Lushchak O., Bagnyukova T., Lushchak V. The effect of aminotriazole on the activity of catalase and glucose-6-phosphate dehydrogenase of two frog species - Rana ridibunda and R. esculenta. Ukr. Biochem. J., 2003, 75 (4), 45-50.

Statistics from Scopus (www.scopus.com): Articles – 33, Citations – 430, h-index – 12.

Reviews and book chapters
Lushchak, V. I., Semchyshyn, H. M. and Lushchak, O. V. (2011) The Classic Methods to Measure Oxidative Damage: Lipid Peroxides, Thiobarbituric-Acid Reactive Substances, and Protein Carbonyls, in Oxidative Stress in Aquatic Ecosystems (eds D. Abele, J. P. Vázquez-Medina and T. Zenteno-Savín), John Wiley & Sons, Ltd, Chichester, UK.


SKILLS
    
General
Experience in article and grant writing, analysis of literature;
Experimental designing;
Individual work or in the group;
Teaching of younger colleagues;
Tissue sampling from fish, frog and mouse;
Mice and fish maintaning

Genetics
E. coli genome manipulations;
Plasmid cloning;
UAS/Gal4 in fruit fly for tissue and organ specific gene overexpression or ablation with drug or temperature induction (gene-switch and temperature sensitivity);

Biochemistry

Assay of enzyme activities (antioxidant and associated, glutathione and carbohydrate metabolism);

Markers of oxidative stress (TBARS, carbonyl proteins, lipid peroxides);

Measurement of fluorescence and chemiluminescense;

Electrophoresis;

Metabolomics (glucose, trehalose, glycogen, TAG, lactate, uric acid, lipid and protein);

Molecular biology
Western blot and ELISA;
Immunohistochemisty;
RT-PCR and qRT-PCR;
Fluorescent and confocal imaging in vivo and in vitro;

Physiology
Fruit fly D. melanogaster and worm C. elegans maintaining;
Fruit fly and worm lifespan assay and analysis;
Fruit fly age-related functional declines (fecundity, mobility, learning ability, sensitivity to stresses, etc)
Fruit fly and larvae learning and memory;

Microbiology
growth (yeast, bacteria);
Selection of transgenes with the use of selective medium;


the contact details of possible references

Dr. Rodney Levine
Principal Investigator  
Laboratory of Biochemistry, NHLBI
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Phone: 301 496 2310
Fax: 301 451 5460
Address: 50 South Dr, Building 50, 2351, MSC-8012         
 Bethesda, MD 20892-8012 USA

Prof. Stephen Simpson

ARC Laureate Fellow


The University of Sydney

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Phone: +61 2 9351 5633
Fax: +61 2 9351 4119
Address: A08 - Heydon-Laurence Building, The University of Sydney, NSW 2006 Australia


Prof. Dr. Dick R. Nässel
Department of Zoology, Stockholm University,
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Phone: +46-8-164077  
Fax: +46-8-167715  
Address: Svante Arrhenius väg 18B, S-106 91 Stockholm, Sweden


Dr. Alberto Sanz
Academy Research Fellow
IBT/Mitochondrial Gerontology and Age-related Diseases, University of Tampere
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Phone: +358 3 3551 8483
Address: Biokatu 6 (Finn-Medi 1), 33520 Tampere, Finland